Journal
NANOTOXICOLOGY
Volume 5, Issue 4, Pages 606-621Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/17435390.2010.541293
Keywords
SPIONs; dendritic cells; immune response; antigen processing; antigen presentation
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Funding
- Department of Clinical Research, Bern University Hospital
- Swiss Society for Pulmonology
- Swiss National Science Foundation [320030-122355, 205321-120161]
- Swiss National Science Foundation (SNF) [320030-122355, 205321-120161] Funding Source: Swiss National Science Foundation (SNF)
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Understanding how nanoparticles may affect immune responses is an essential prerequisite to developing novel clinical applications. To investigate nanoparticle-dependent outcomes on immune responses, dendritic cells (DCs) were treated with model biomedical poly(vinylalcohol)-coated super-paramagnetic iron oxide nanoparticles (PVA-SPIONs). PVA-SPIONs uptake by human monocyte-derived DCs (MDDCs) was analyzed by flow cytometry (FACS) and advanced imaging techniques. Viability, activation, function, and stimulatory capacity of MDDCs were assessed by FACS and an in vitro CD4(+) T cell assay. PVA-SPION uptake was dose-dependent, decreased by lipopolysaccharide (LPS)-induced MDDC maturation at higher particle concentrations, and was inhibited by cytochalasin D pre-treatment. PVA-SPIONs did not alter surface marker expression (CD80, CD83, CD86, myeloid/plasmacytoid DC markers) or antigen-uptake, but decreased the capacity of MDDCs to process antigen, stimulate CD4(+) T cells, and induce cytokines. The decreased antigen processing and CD4(+) T cell stimulation capability of MDDCs following PVA-SPION treatment suggests that MDDCs may revert to a more functionally immature state following particle exposure.
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