Journal
NANOTOXICOLOGY
Volume 5, Issue 3, Pages 284-295Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/17435390.2010.512401
Keywords
Gold nanoparticles; macrophages; cytotoxicity; inflammation; cytokine
Categories
Funding
- U.S. Food and Drug Administration
- Oak Ridge Research Fellowship Program (ORISE) Associated University through a contract with the U.S. Food and Drug Administration
Ask authors/readers for more resources
More information characterizing the biological responses to nanoparticles is needed to allow the U. S. Food and Drug Administration to evaluate the safety and effectiveness of products with nano-scale components. The potential cytotoxicity and inflammatory responses of Au NPs (60 nm, NIST standard reference materials) were investigated in murine macrophages. Cytotoxicity was evaluated by MTT and LDH assays. Cytokines (IL-6, TNF-alpha), nitric oxide, and ROS were assayed to assess inflammatory responses. Morphological appearance and localization of particles were examined by high resolution illumination microscopy, transmission electron microscopy (TEM), and scanning TEM coupled with EDX spectroscopy. Results showed no cytotoxicity and no elevated production of proinflammatory mediators; however, imaging analyses demonstrated cellular uptake of Au NPs and localization within intracellular vacuoles. These results suggest that 60 nm Au NPs, under the exposure conditions tested, are not cytotoxic, nor elicit pro-inflammatory responses. The localization of Au NPs in intracellular vacuoles suggests endosomal containment and an uptake mechanism involving endocytosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available