Journal
NANOTOXICOLOGY
Volume 3, Issue 4, Pages 307-318Publisher
INFORMA HEALTHCARE
DOI: 10.3109/17435390903121949
Keywords
Nanoparticles; exposure
Categories
Funding
- NSF
- DoD MURI [FA9550-04-1-0430]
- NIH [RO1 ES10041, RO1 CA134218]
- DoD MHRP [W81 XWH-05-1-0239]
- NIEHS Center [P30ES01247]
- NATIONAL CANCER INSTITUTE [R01CA134218] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R01ES010041, P30ES001247] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Mammalian cells take up nanoparticles (NPs) and some NPs increase ROS. We used imaging and measure ROS in parallel to evaluate NP-cell interactions with type I-like alveolar epithelial cells exposed to NPs at 1.2 mu g/cm(2). Titanium dioxide (TiO(2)), gold (Au), silver (Ag), and manganese (Mn) were internalized by R3-1 cells; copper (Cu) NPs were observed at the cell surface only. TiO(2) and Au did not increase cell death but Mn and Cu did, with surviving cells recovering after initial Cu exposure. Ag NPs caused 80% of R3-1 cells to lift off the slides within 1 h. Amplex Red was used to report H(2)O(2) production after exposure to 0.4 mu g/cm(2) TiO(2), Au, Cu, Mn and Ag. TiO(2), Au, and Ag caused no significant increase in H(2)O(2) while Cu and Mn increased H(2)O(2). NPs that give up electrons, increase ROS production and cause cell death in R3-1 cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available