Journal
NANOTECHNOLOGY
Volume 24, Issue 49, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/0957-4484/24/49/495102
Keywords
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Funding
- FIU CAS
- DNA Sequencing Technology Program of the National Human Genome Research Institute [1RC2HG005625-01, 1R21HG004770-01]
- China NSFC [21073181]
- China CAS '100 Talent Program'
- Scientific User Facilities Division, Office of Basic Energy Sciences, US Department of Energy
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Studies of DNA translocation through graphene nanopores have revealed their potential for DNA sequencing. Here we report a study of protein translocation through chemically modified graphene nanopores. A transmission electron microscope (TEM) was used to cut nanopores with diameters between 5 and 20 nm in multilayer graphene prepared by chemical vapor deposition (CVD). After oxygen plasma treatment, the dependence of the measured ionic current on salt concentration and pH was consistent with a small surface charge induced by the formation of carboxyl groups. While translocation of gold nanoparticles (10 nm) was readily detected through such treated pores of a larger diameter, translocation of the protein ferritin was not observed either for oxygen plasma treated pores, or for pores modified with mercaptohexadecanoic acid. Ferritin translocation events were reliably observed after the pores were modified with the phospholipid-PEG (DPPE-PEG750) amphiphile. The ion current signature of translocation events was complex, suggesting that a series of interactions between the protein and pores occurs during the process.
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