Journal
SEPARATION SCIENCE AND TECHNOLOGY
Volume 51, Issue 2, Pages 359-369Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/01496395.2015.1086375
Keywords
Deproteinization; exopolysaccharide; Ganoderma sinense mycelium; protein-bound polysaccharide; immunostimulation
Funding
- National Natural Science Foundation of China [30800193]
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Deproteinization was comparatively employed for the purification of crude exopolysaccharide (CEP) of Ganoderma sinense mycelium. Results showed that ammonium sulfate (70% saturation) salting out could remove 95% proteins of CEP, whereas 20% trichloroacetic acid precipitation was best with a polysaccharide loss less than 20%. Protein-bound polysaccharides could be removed by both methods, and polysaccharides with high molecular weight dominated in the lost polysaccharides. Through anion-exchange chromatography, a highly branched -(16)-D-mannan and a protein-bound heteropolysaccharide were prepared from CEP. Both fractions could inhibit the in vitro proliferation of tumor cells BEL-7402, and induce TNF- secretion of murine splenocytes.
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