Journal
NANOTECHNOLOGY
Volume 24, Issue 2, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/0957-4484/24/2/025605
Keywords
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Funding
- UK EPSRC [EP/H007334/1, EP/C006755/1]
- University of Leeds Institute of Bionanosciences
- Wellcome Trust [[062164][090932/Z/09/Z]]
- Royal Society
- Wolfson Foundation
- EPSRC [EP/K023845/1, EP/E015530/1, EP/I000623/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/C006755/1] Funding Source: researchfish
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The rod-shaped plant virus tobacco mosaic virus (TMV) is widely used as a nano-fabrication template, and chimeric peptide expression on its major coat protein has extended its potential applications. Here we describe a simple bacterial expression system for production and rapid purification of recombinant chimeric TMV coat protein carrying C-terminal peptide tags. These proteins do not bind TMV RNA or form disks at pH 7. However, they retain the ability to self-assemble into virus-like arrays at acidic pH. C-terminal peptide tags in such arrays are exposed on the protein surface, allowing interaction with target species. We have utilized a C-terminal His-tag to create virus coat protein-templated nano-rods able to bind gold nanoparticles uniformly. These can be transformed into gold nano-wires by deposition of additional gold atoms from solution, followed by thermal annealing. The resistivity of a typical annealed wire created by this approach is significantly less than values reported for other nano-wires made using different bio-templates. This expression construct is therefore a useful additional tool for the creation of chimeric TMV-like nano-rods for bio-templating.
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