4.6 Article

Effects of buffer composition and dilution on nanowire field-effect biosensors

Journal

NANOTECHNOLOGY
Volume 24, Issue 3, Pages -

Publisher

IOP PUBLISHING LTD
DOI: 10.1088/0957-4484/24/3/035501

Keywords

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Funding

  1. Lundbeck Foundation
  2. Danish Agency for Science Technology and Innovation [FNU 272-05-0355, FTP 274-07-0277, NABIIT 2106-05-0036]
  3. UNIK Synthetic Biology
  4. Danish Ministry for Science, Technology and Innovation

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Nanowire-based field-effect transistors (FETs) can be used as ultra-sensitive and label-free biosensors for detecting protein-protein interactions. A way to increase the performance of such sensors is to dilute the sensing buffer drastically. However, we show here that this can have an important effect on the function of the proteins. Moreover, it is demonstrated that this dilution significantly affects the pH stability of the sensing buffer, which consequently impacts the charge of the protein and thus the response and signal-to-noise ratio in the sensing experiments. Three model systems are investigated experimentally to illustrate the impact on ligand-protein and protein-protein interactions. Simulations are performed to illustrate the effect on the performance of the sensors. Combining various parameters, the current study provides a means for evaluating and selecting the most appropriate buffer composition for bioFET measurements. S Online supplementary data available from stacks.iop.org/Nano/24/035501/mmedia

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