Journal
NANOTECHNOLOGY
Volume 22, Issue 27, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/0957-4484/22/27/275103
Keywords
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Funding
- Andalusian Regional Ministry of Health the 'Andalusian Initiative for Advanced Therapies'-Fundacion Progreso y Salud
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Human transferrin has been biolabelled with gold nanoclusters (Au NCs) using a simple, fast and non-toxic method. These nanocrystals (<2 nm) are stabilized in the protein via sulfur groups and have a high fluorescence emission in the near infrared region (QY = 4.3%; lambda(em) = 695 nm). Structural investigation and photophysical measurements show a high population of clusters formed of 22-33 gold atoms covalently bound to the transferrin. In solutions with pH ranging from 5 to 10 and in buffer solutions (PBS, HEPES), those biolabelled proteins exhibit a good stability. No significant quenching effect of the fluorescent transferrin has been detected after iron loading of iron-free transferrin (apoTf) and in the presence of a specific polyclonal antibody. Additionally, antibody-induced agglomeration demonstrates no alteration in the protein activity and the receptor target ability. MTT and Vialight (R) Plus tests show no cytotoxicity of these labelled proteins in cells (1 mu g ml(-1)-1 mg ml(-1)). Cell line experiments (A549) indicate also an uptake of the iron loaded fluorescent proteins inside cells. These remarkable data highlight the potential of a new type of non-toxic fluorescent transferrin for imaging and targeting.
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