Journal
SEPARATION AND PURIFICATION TECHNOLOGY
Volume 149, Issue -, Pages 116-123Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.seppur.2015.03.037
Keywords
Polyols-based deep eutectic solvent; Pyrola incarnata Fisch.; Phenolics; Sample preparation; Determination
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Funding
- Key Program for Application Technology Research and Development Program of Harbin [2013AA3BS014]
- Fundamental Research Funds for the Central Universities [2572014AA13]
- Special Fund of National Natural Science Foundation of China [31270618]
- Importation of International Advanced Forestry Science and Technology, National Forestry Bureau [2012-4-06]
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A group of green solvent named polyols-based deep eutectic solvents (PDES) combined with microwave assisted extraction (MAE) was first successfully applied in extracting phenolic compounds from pyrola. A series of PDES with mixing different ratios choline chloride (ChCl) to different polyols were investigated for extraction, and ChCl/1,4-Butanediol (1/4) was found to be the suitable extraction solvent. MAE was more effective than heat-stirring extraction and ultrasonic assisted extraction with PDES. The extraction parameters were optimized by BBD test combined with response surface methodology. The optimal extraction conditions were 30% of water in ChCl/1,4-Butanediol (1/4) at 70 degrees C for 20 min and liquid/solid ratio 10 mL/g. Moreover, RP-HPLC-UV was developed for the determination of five main active components in pyrola. This method exhibited satisfactory linearity (R-2 > 0.999), precision (RSD < 4.03%) and recovery (96.17-103.25%). Sample analysis showed that the contents of hyperin, 2'-O-galloylhyperin, quercitrin, quercetin-O-rhamnoside and chimaphilin in pyrola were 1.627, 4.958, 0.041, 0.089 and 0.349 mg/g, respectively. These results revealed that PDES would be promising green solvents for analytical sample preparation from plant herbs. (C) 2015 Elsevier B.V. All rights reserved.
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