Journal
NANOTECHNOLOGY
Volume 21, Issue 11, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/0957-4484/21/11/115103
Keywords
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Funding
- National Natural Science Foundation of China [20974062, 50773037, 50633010]
- National Basic Research Program [2007CB808000]
- Fok Ying Tung Education Foundation [111048]
- Science and Technology Commission of Shanghai Municipality [0852nm05800]
- Shanghai Leading Academic Discipline Project [B202]
- Shuguang Program [08SG14]
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Magnetic nonviral gene vectors were in situ prepared in the presence of ferrous salts and hyperbranched poly(ethylenimine)s (HPEI) with different molecular weights. HPEI, one of the most promising nonviral vectors, was not only utilized as the nanoreactor and stabilizer to prepare magnetic nanoparticles, but also skillfully used as a base supplier to avoid introducing alkali hydroxide or ammonia. Magnetic nonviral gene vectors with various magnetite contents and saturation magnetizations were obtained by changing the weight ratio of HPEI to FeSO4 center dot 7H(2)O and the molecular weight of HPEI. MTT assays suggested that the resulting magnetite/HPEI gene vectors had lower cytotoxicity compared with pure HPEI. The magnetite/HPEI nonviral gene vectors were used for magnetofection. It was found that the luciferase expression level mediated by magnetite/HPEI in COS-7 cells under a magnetic gradient field was approximately 13-fold greater than that of standard HPEI transfection.
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