Journal
NANOTECHNOLOGY
Volume 20, Issue 43, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/0957-4484/20/43/434004
Keywords
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Funding
- National Institutes of Health [GM077173]
- NSF [CHE-0808945]
- MRSEC
- Center for Hierarchical Manufacturing [DMI-0531171]
- NSF for an IGERT [DUE-044852]
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Tetraethylene glycol (TEG) functionalized gold nanoparticles with 2 nm core diameters (AuTEG) enhance alpha-chymotrypsin (ChT) enzyme activity in a substrate-selective fashion. We explored the hydrolysis of four different substrates and observed a marked increase in activity with the most hydrophobic substrate N-succinyl-alanine-alanine-proline-phenylalanine-p-nitroanilide (TP), while the other substrates remain virtually unaffected by the AuTEG 'crowding effect' in solution. The enhancement in catalysis is indicated by an increase in K-cat/K-m as obtained from Lineweaver-Burk analysis and we hypothesize it to arise from a macromolecular crowding effect analogous to that observed with high molecular weight poly(ethylene glycol) (PEG) polymers.
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