Journal
NANOTECHNOLOGY
Volume 19, Issue 41, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/0957-4484/19/41/415605
Keywords
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Funding
- National University of Singapore [ARF R-279-000-197-112/133, YIA R-279-000234-123]
- Singapore Ministry of Education [R-279-000255-112]
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Novel methods for rapid, sensitive and low-cost biomolecule detection have attracted particular interest because of their wide use in medical diagnostics, food inspection and biomedical research applications. In this work, we report a simple and efficient silica nanoparticle (NP)-based fluorescent assay for ATP detection. It takes advantage of the washing and separation properties of NPs and the structure-switch property of DNA aptamers, resulting in fluorescence change of the supernatant in the presence of targets. A linear response for ATP detection was observed from 0 to 6 mM with a detection limit of similar to 34 mu M. This detection strategy could be generalized to other aptamer-based detection systems.
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