Journal
NANOSCALE RESEARCH LETTERS
Volume 4, Issue 4, Pages 287-295Publisher
SPRINGER
DOI: 10.1007/s11671-008-9239-9
Keywords
Magnetic nanoparticle; Fluorescence; Chitosan; Magnetic resonance imaging
Funding
- National Natural Science Foundation of China [60571031, 60501009, 90406023]
- National Basic Research Program of China [2006CB933206, 2006CB705600]
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Labeling of cells with nanoparticles for living detection is of interest to various biomedical applications. In this study, novel fluorescent/magnetic nanoparticles were prepared and used in high-efficient cellular imaging. The nanoparticles coated with the modified chitosan possessed a magnetic oxide core and a covalently attached fluorescent dye. We evaluated the feasibility and efficiency in labeling cancer cells (SMMC-7721) with the nanoparticles. The nanoparticles exhibited a high affinity to cells, which was demonstrated by flow cytometry and magnetic resonance imaging. The results showed that cell-labeling efficiency of the nanoparticles was dependent on the incubation time and nanoparticles' concentration. The minimum detected number of labeled cells was around 10(4) by using a clinical 1.5-T MRI imager. Fluorescence and transmission electron microscopy instruments were used to monitor the localization patterns of the magnetic nanoparticles in cells. These new magneto-fluorescent nanoagents have demonstrated the potential for future medical use.
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