Journal
NANOSCALE RESEARCH LETTERS
Volume 4, Issue 3, Pages 216-220Publisher
SPRINGER
DOI: 10.1007/s11671-008-9228-z
Keywords
Single-stranded DNA; HCl; Gold nanoparticles; p53; Mutation
Funding
- Science and Technology Innovation Project of Fujian Province for Young Scientific Researchers, China [2006F3128]
- Open Fund of State Key Laboratory for Physical Chemistry of Solid Surfaces, Xiamen University [200602]
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Gold nanoparticles (GNPs) are widely used to detect DNA. We studied the effect of pH on the assembly/disassembly of single-stranded DNA functionalized GNPs. Based on the different binding affinities of DNA to GNPs, we present a simple and fast way that uses HCl to drive the assembly of GNPs for detection of DNA sequences with single nucleotide differences. The assembly is reversible and can be switched by changing the solution pH. No covalent modification of DNA or GNP surface is needed. Oligonucleotide derived from human p53 gene with one-base substitution can be distinguished by a color change of the GNPs solution or a significant difference of the maximum absorption wavelength (lambda(max)), compared with wildtype sequences. This method enables detection of 10 picomole quantities of target DNA.
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