Journal
NANOSCALE
Volume 10, Issue 6, Pages 2825-2833Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7nr06633c
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Funding
- National Natural Science Foundation of China [81673172, 21475144, 91227116, 21705087]
- National Institute of Health [5R01CA125063-05]
- NATIONAL CANCER INSTITUTE [R01CA125063] Funding Source: NIH RePORTER
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Pathogen detection continues to receive significant attention due to the harmful effects of pathogens on public health. Herein, specific nonapeptide-fusion proteins pVIII (pVIII fusion) were isolated from phage VQTVQIGSD (designated by the sequence of a fused foreign peptide), which was specifically screened from the f8/9 landscape phage library against Vibrio parahaemolyticus (V. parahaemolyticus) in a highthroughput way. The as-prepared V. parahaemolyticus-specific recognition element is cheaper and more available than antibodies. Further, a highly sensitive colorimetric immunoassay for V. parahaemolyticus was established using pVIII fusion as capture probes coupled with protein-templated MnO2 nanosheets (NSs) as signal probes. In the presence of a target bacterium, V. parahaemolyticus, a sandwich-type complex of pVIII fusion-V. parahaemolyticus-MnO2 NS@pVIII fusion was formed through specific recognition of pVIII fusion and V. parahaemolyticus. The signal probes (MnO2 NSs) could catalyze the reaction of 3,3', 5,5'-tetramethylbenzidine and H2O2 to generate a colorimetric change. The proposed V. parahaemolyticus detection method demonstrated a wide detection range (20-10(4) colony-forming units (CFU) mL(-1)), low limit of detection (15 CFU mL(-1)), excellent selectivity, and high reliability for real marine samples, showing potential application in marine microbiological detection and control.
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