Journal
NANOSCALE
Volume 6, Issue 7, Pages 3804-3809Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3nr06549a
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Funding
- National Natural Science Foundation of China [21236003, 21206042, 20925621, 21176083]
- Basic Research Program of Shanghai [13NM1400700, 13NM1400701]
- Fundamental Research Funds for the Central Universities
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Here, we report a simple and ultrasensitive upconversion fluorescent strip sensor based on NaYF4:Yb, Er nanoparticles (NPs) and the lateral flow immunochromatographic assay (LFIA). Carboxyl-modified b-NaYF4:Yb, Er NPs were successfully synthesized by a facile one-pot solvothermal approach, upon further coupling with monoclonal antibody, the resultant UCNPs-antibody conjugates probes were used in LFIA and served as signal vehicles for the fluorescent reporters. V. anguillarum was used as a model analyte to demonstrate the use of this strip sensor. The limit of the detection for the fluorescent strip was determined as 10(2) CFU mL(-1), which is 100 times lower than those displayed by enzyme-linked immunosorbent assays, while the time needed for the detection was only 15 min. Furthermore, no cross-reaction with other eight pathogens was found, indicating the good specificity of the strip. This developed LFIA would offer the potential as a useful tool for the quantification of pathogens analysis in the future.
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