Journal
NANOSCALE
Volume 6, Issue 10, Pages 5315-5322Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4nr00355a
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Funding
- National Natural Scientific Foundation of China [21201092, 51203184]
- Research Fund for the Doctoral Program of Higher Education [20120211120020]
- Gansu NST [1208RJYA028]
- Fundamental Research Funds for the Central Universities [Lzujbky-2012-213, Lzujbky-2012-65, lzujbky-2012-189]
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We report a facile modification of graphene oxide (GO) by gelatin to mimic charged proteins present in the extracellular matrix during bone formation. The bioinspired surface of GO-gelatin (GO-Gel) composite was used for biomimetic mineralization of hydroxyapatite (HA). A detailed structural and morphological characterization of the mineralized composite was performed. Additionally, MC3T3-E1 cells were cultured on the GO-Gel surfaces to observe various cellular activities and HA mineralization. Higher cellular activities such as cell adhesion, cell proliferation, and alkaline phosphatase activity (ALP) were observed on the GO-Gel surface compared with the GO or glass surface. The increase of ALP confirms that the proposed GO-Gel promotes the osteogenic differentiation of MC3T3-E1 cells. Moreover, the evidence of mineralization evaluated by scanning electron microscopy (SEM) and alizarin red staining (ARS) corroborate the idea that a native osteoid matrix is ultimately deposited. All these data suggest that the GO-Gel hybrids will have great potential as osteogenesis promoting scaffolds for successful application in bone surgery.
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