Journal
NANOSCALE
Volume 4, Issue 2, Pages 451-454Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2nr11657j
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Funding
- NSFC [20875087]
- Jilin Provincial Science and Technology Department [20100701]
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Herein, we report a phenylboronic acid functionalized gold nanoparticle (GNP)-based colorimetric assay for rapid detection of Staphylococcus aureus (S. aureus) with high sensitivity. In this approach, GNPs can bind to S. aureus by the reaction of phenylboronic acid with the cis-diol configuration in glycans on the bacterial surface, providing a colorimetric readout of the binding event. Using this strategy, we have been able to quantify S. aureus at a concentration of 50 cells per mL (three times the standard deviation divided by the slope of the working curve) in aqueous solution.
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