Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 220, Issue -, Pages 727-733Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.06.013
Keywords
Fluorescent chemodosimeter; Nitroxyl; 2-(2 '-Hydroxyphenyl)benzothiazole; 2-(Diphenylphosphino)benzoate; Bioimaging
Funding
- National Natural Science Foundation of China [21107029]
- Outstanding Young Scientists Award Fund of Shandong Province [B52013HZ007]
Ask authors/readers for more resources
Nitroxyl (HNO) has attracted increasing attention for its ability to enhance myocardial contractility and vasorelaxation in heart failure. However, studies of the detailed functions and mechanisms of HNO in vivo are hampered by a lack of reliable detection methods. Herein, we report a novel 2-(diphenylphosphino)benzoate-caged 2-(2'-hydroxyphenyl)benzothiazole fluorescent chemodosimter (HNO-HBT) for the sensitive and rapid detection of HNO in living cells. HNO-HBT exhibits high HNO-selectivity even in the presence of high concentration of biological reductants including glutathione (GSH), hydrogen sulfide (H2S) and ascorbate (AA), which is ascribed to the adoption of the 2-(diphenylphosphino)benzoate recognition moiety. In addition, the results showed that HNO-HBT could quantitatively detect HNO in the range of 0-40 mu M with the detection limit of 50 nM. Importantly, HNO-HBT was successfully applied to the fluorescence imaging of HNO levels in living cells, and it is expected to be a useful chemical tool for investigating the detailed functions and mechanisms of HNO in living systems. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available