Journal
NANOSCALE
Volume 2, Issue 4, Pages 495-498Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b9nr00236g
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Funding
- Universidad Autonoma de Madrid ad Comunidad Autonoma de Madrid [CCG07-UAM/MAT-18611]
- Spanish Ministerio de Educacion y Ciencia [MAT 2007-64686]
- Banco Santander-CEAL-UAM
- Natural Sciences and Engineering Research Council (NSERC) of Canada
- Gouvernement du Quebec
- Ministere du Developpement economique
- de l'Innovation et de l'Exportation
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We report on the efficient incorporation of non-functionalized NaYF4 : Er3+, Yb3+ nanoparticles inside HeLa live cancer cells by direct endocytosis. The efficient two-photon excited near-infrared-to-visible upconversion fluorescence of these nanoparticles is then used to obtain high-contrast intracellular fluorescence images of single cells. These images reveal a redistribution of the nanoparticles inside the cell as the incubation time increases. Thus, non-functionalized NaYF4 : Er3+, Yb3+ nanoparticles emerge as very promising fluorescence probes for real-time imaging of cellular dynamics.
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