Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 10, Issue 7, Pages 1571-1581Publisher
ELSEVIER
DOI: 10.1016/j.nano.2014.04.004
Keywords
Tape stripping; Allergic contact dermatitis; Murine model; FLIM; Fluorescence microscopy
Funding
- German Research Foundation (DFG) Priority Program 1313 Biological Responses to Nanoscale Particles
- Leibniz Graduate School of Molecular Biophysics
- Helmholtz Virtual Institute on Multifunctional Biomaterials for Medicine
- DFG [SFB 1112, B02, B03, C03]
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The skin is a potential site of entry for nanoparticles (NP) but the role of disease-associated barrier disturbances on the path and extent of skin penetration of NP remains to be characterized. Silica nanoparticles (SiO2-NP) possess promising potential for various medical applications. Here, effects of different skin barrier disruptions on the penetration of N-(6-aminohexyl)-aminopropyltrimethoxysilane (AHAPS) functionalized SiO2-NP were studied. AHAPS-SiO2-NP (55 +/- 6 nm diameter) were topically applied on intact, tape stripped or on inflamed skin of SKH1 mice with induced allergic contact dermatitis for one or five consecutive days, respectively. Penetration of AHAPS-SiO2-NP through the skin was not observed regardless of the kind of barrier disruption. However, only after subcutaneous injection, AHAPS-SiO2-NP were incorporated by macrophages and transported to the regional lymph node only. Adverse effects on cells or tissues were not observed. In conclusion, AHAPS-SiO2-NP seem to not cross the normal or perturbed mouse skin. From the Clinical Editor: Skin is a potential site of entry for nanoparticles; however, it is poorly understood how skin diseases may alter this process. In tape-stripped skin and allergic contact dermatitis models the delivery properties of AHAPS-SiO2 nanoparticles remained unchanged, and in neither case were these NP-s able to penetrate the skin. No adverse effects were noted on the skin in these models and control mice. (C) 2014 Elsevier Inc. All rights reserved.
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