Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 10, Issue 8, Pages 1863-1870Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2014.04.010
Keywords
Pseudomonas aeruginosa; Pyocyanin; Surface-enhanced Raman spectroscopy; Silver nanorod arrays; Biomarker
Funding
- National Institute of Health [R21-AI096364]
- National Science Foundation [CBET-1064228]
- Emory-Egleston Children Research Center
- Center for Pediatric Nanomedicine of the Emory + Children's Pediatric Research Center
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1064228] Funding Source: National Science Foundation
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Pseudomonas aeruginosa can causemajor infection in immunocompromised patients, and successful antibiotic treatment of the infection relies on accurate and rapid identification of the infectious agents. Here, we reported a culture-free diagnostic method based on the surface-enhanced Raman spectroscopy (SERS) of pyocyanin (PCN), amajor biomarker of P. aeruginosa. This platform can detect PCN as low as 5 ppb or 2.38x10(-8) mol L-1 in both aqueous solutions and spiked clinical sputum samples. It has also been used to dynamically monitor the excretion of PCN by P. aeruginosa during its growth. The presence of PCN has been detected by SERS in 15 clinical sputum samples, which indicates P. aeruginosa infection, with 95.6% sensitivity and 93.3% specificity. The system can advantageously process multiple specimens rapidly, overcomes the need for bacterial culture and diagnostic microbiology assays, and have widespread implications in the early detection of P. aeruginosa infection. From the Clinical Editor: A surface enhanced Raman spectroscopy method optimized for the detection of P. aureginosa infections is presented in this paper. The presence of pyocyanin, a marker of this bacterium has been detected in 15 clinical sputum samples utilizing this method. A sensitivity of 95.6% and 93.3% specificity was reported, which suggests that the method may enable culture-free high throughput rapid detection of this infection. (C) 2014 Elsevier Inc. All rights reserved.
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