Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 8, Issue 3, Pages 355-364Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2011.06.021
Keywords
Actin fibers; Idiopathic pulmonary fibrosis; Extracellular matrix; Scanning probe microscopy; A549 cell line
Funding
- IRCSET Government of Ireland
- Strategic Research Cluster grant under the National Development Plan [07/SRC/B1154]
- EU
- Science Foundation Ireland [07/IN1/B931]
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Epithelial-mesenchymal transition (EMT) is closely implicated in the pathogenesis of idiopathic pulmonary fibrosis. Associated with this phenotypic transition is the acquisition of an elongated cell morphology and establishment of stress fibers. The extent to which these EMT-associated changes influence cellular mechanics is unclear. We assessed the biomechanical properties of alveolar epithelial cells (A549) following exposure to TGF-beta 1. Using atomic force microscopy, changes in cell stiffness and surface membrane features were determined. Stimulation with TGF-beta 1 gave rise to a significant increase in stiffness, which was augmented by a collagen I matrix. Additionally, TGF-beta 1-treated cells exhibited a rougher surface profile with notable protrusions. Simultaneous quantitative examination of the morphological attributes of stimulated cells using an image-based high-content analysis system revealed dramatic alterations in cell shape, F-actin content and distribution. Together, these investigations point to a strong correlation between the cytoskeletal-associated cellular architecture and the mechanical dynamics of alveolar epithelial cells undergoing EMT.
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