Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 6, Issue 1, Pages 119-126Publisher
ELSEVIER
DOI: 10.1016/j.nano.2009.06.006
Keywords
beta-Casein micelles; Cancer; Mitoxantrone; Nanoparticles; Oral delivery
Funding
- Yehuda and Civana Kahani
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Bovine beta-casein (beta-CN) is an abundant milk protein that is highly amphiphilic and self-assembles into stable micellar structures in aqueous solutions. Here we introduce a drug-delivery system comprising a model hydrophobic anticancer drug, mitoxantrone (MX), entrapped within beta-CN-based nanoparticles. This novel drug-delivery system allows hydrophobic drugs to be thermodynamically stable in aqueous solutions for oral-delivery applications aimed at treatment of various disorders. The gastric digestibility of beta-CN suggests possible targeting to stomach tumors. Dimethyl sulfoxide (DMSO)-dissolved MX was entrapped in beta-CN nanoparticles by stirring this solution into phosphate-buffered beta-CN solution. High-affinity MX-beta-CN association was found (K-a = [2.15 +/- 0.30] x 10(6) M-1). The optimal nanovehicle formation conditions were 1 mg/mL beta-CN, = 6% (vol/vol) DMSO in phosphate-buffer solution, 10 mM MX in DMSO, and a MX: beta-CN molar-ratio of similar to 4:1. Under these conditions, particles of 100 to 300-nm diameter were formed. beta-CN nanoparticles may serve as effective oral-delivery nanovehicles for solubilization and stabilization of hydrophobic drugs. From the Clinical Editor: Bovine beta-casein (beta-CN) is an abundant milk-protein that is highly amphiphilic and self-assembles into stable micellar-structures in aqueous solutions. beta-CN nanoparticles may serve as effective oral-delivery nanovehicles for solubilization and stabilization of hydrophobic drugs, as demonstrated in this study utilizing methotrexate. (C) 2010 Elsevier Inc. All rights reserved.
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