Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 6, Issue 4, Pages 597-603Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2009.12.004
Keywords
Nanosuspension; Amphotericin B; Two-dimensional polyacrylamide gel electrophoresis; In vitro-in vivo correlation; High-pressure homogenization; Balamuthia mandrillaris
Funding
- European Commission [QLRT-2000-01404]
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Amphotericin B was formulated as nanosuspensions to develop a nanoparticulate brain delivery system. Nanosuspensions were produced with different surfactant solutions by high-pressure homogenization and then characterized by laser diffractometry and photon correlation spectroscopy. Before in vitro and in vivo testing all nanosuspensions were investigated for protein adsorption by two-dimensional polyacrylamide gel electrophoresis to predict brain-targeting capacities. Selected nanosuspensions were tested for amebicidal activity against Balamuthia mandrillaris, an agent of lethal encephalitis. Our results indicate that nanosuspensions coated with polysorbate 80 and sodium cholate markedly increased drug brain delivery and inhibited the parasite in vitro, though less in vivo. From the Clinical Editor: The antifungal Amphotericin B was formulated as nanosuspensions to develop a nanoparticulate brain delivery system. The results indicate that nanosuspensions coated with polysorbate 80 and sodium cholate markedly increased drug brain delivery and inhibited the parasite in vitro, though less in vivo. (C) 2010 Elsevier Inc. All rights reserved.
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