Journal
NANOMEDICINE
Volume 9, Issue 2, Pages 207-220Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/nnm.13.43
Keywords
inclusion body; mesenchymal stem cell; metabolomic; topography
Funding
- Biotechnology and Biological Sciences Research Council [BB/G008868/1]
- MICINN [BFU2010-17450]
- CIBER de Bioingenieria, Biomateriales y Nanomedicina, Spain
- European Molecular Biology Organization [ASTF 359.00-2011]
- Universitat Autonoma de Barcelona
- Programa Personal de Tecnico de Apoyo (Modalidad Infraestructuras Cientifico-Tecnologicas, Ministerio de Ciencia e Innovacion)
- Biotechnology and Biological Sciences Research Council [BB/G008868/1] Funding Source: researchfish
- BBSRC [BB/G008868/1] Funding Source: UKRI
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Aim: Bacterial inclusion bodies (IBs) are nanostructured (submicron), pseudospherical proteinaceous particles produced in recombinant bacteria resulting from ordered protein aggregation. Being mechanically stable, several physicochemical and biological properties of IBs can be tuned by appropriate selection of the producer strain and of culture conditions. It has been previously shown that IBs favor cell adhesion and surface colonization by mammalian cell lines upon decoration on materials surfaces, but how these biomaterials could influence the behavior of mesenchymal stem cells remains to be explored. Materials & methods: Here, the authors vary topography, stiffness and wettability using the IBs to decorate polycaprolactone surfaces on which mesenchymal stem cells are cultured. Results: The authors show that these topographies can be used to specifically target osteogenesis from mesenchymal stem cells, and through metabolomics, they show that the cells have increased energy demand during this bone-related differentiation. Conclusion: IBs as topographies can be used not only to direct cell proliferation but also to target differentiation of mesenchymal stem cells. Original submitted 12 October 2012; Revised submitted 22 January 2013; Published online 30 April 2013
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