Journal
NANOMEDICINE
Volume 9, Issue 9, Pages 1327-1339Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/NNM.14.48
Keywords
bronchial alveolar lavage fluid; macrophage; nitric oxide; nontypeable Haemophilus influenzae; zinc oxide nanoparticles
Funding
- National Science Council [NSC101-2313-B-039-004-MY3, NSC102-2314-B-039-016, NSC102-2633-B-039-001]
- China Medical University, Taiwan [CMU102-S-27, CMU102-S-28]
- Taiwan Ministry of Health and Welfare Clinical Trial, the Research Center for Excellence [DOH103-TD-B-111-004]
- Tomorrow Medicine Foundation
Ask authors/readers for more resources
Aim: The extensive development of nanoparticles (NPs) and their widespread employment in daily life have led to an increase in environmental concentrations of substances that may pose a biohazard to humans. The aim of this work was to examine the effects of zinc oxide nanoparticles (ZnO-NPs) on the host's pulmonary immune system response to nontypeable Haemophilus influenzae (NTHi) infection. Materials & Methods: A murine infection model was employed to assess pulmonary inflammation and bacterial clearance in response to exposure to ZnO-NPs. The molecular mechanisms underlying ZnO-NP-impaired macrophage activation were investigated. Results: Treatment with ZnO-NPs impaired macrophage activation, leading to a delay in NTHi clearance in the bronchial alveolar lavage fluids and lungs. Exposure to ZnO-NPs followed by NTHi challenge decreased levels of nitric oxide compared with NTHi infection alone. The effects of ZnO-NPs involved downregulation of NTHi-activated expression of inducible nitric oxide synthase and the translocation of active NF-kB into the nucleus. Conclusion: These results demonstrate that exposure to ZnO-NPs can impair innate immune responses and attenuate macrophage responses to bacterial infection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available