Journal
NANOMEDICINE
Volume 5, Issue 1, Pages 77-88Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/NNM.09.92
Keywords
AAO; anodic aluminum oxide; barcode; bioassay; cancer marker; magnetic decoding; multiplex assay; silica nanotubes; suspension array
Funding
- University of Maryland
- NIH
- Korean government [MEST R31-2008-000-10071-0]
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [R01DE019050] Funding Source: NIH RePORTER
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Aims: The recent development of 1D barcode arrays has proved their capabilities to be applicable to highly multiplexed bioassays. This article introduces two magnetic decoding protocols for suspension arrays of shape-coded silica nanotubes to process multiplexed assays rapidly and easily, which will benefit the minimization and automation of the arrays. Methods: In the first protocol, the magnetic nanocrystals are incorporated into the inner voids of barcoded silica nanotubes in order to give the nanotubes magnetic properties. The second protocol is performed by trapping the barcoded silica nanotubes onto streptavidin-modified magnetic beads. Results: The rapid and easy decoding process was demonstrated by applying the above two protocols to multiplexed assays, resulting in high selectivity. Furthermore, the magnetic bead-trapped barcode nanotubes provided a great opportunity to exclude the use of dye molecules in multiplexed assays by using barcode nanotubes as signals. Conclusion: The rapid and easy manipulation of encoded carriers using magnetic properties could be used to develop promising suspension arrays for portable bioassays.
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