Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 214, Issue -, Pages 124-131Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.03.021
Keywords
Sandwich-type electrochemical immunosensor; Tissue polypeptide antigen; Ferriferrous oxide nanoparticles; Gold nanoparticles; Graphene
Funding
- National Natural Science Foundation of China [21175057, 21375047, 21377046, 21405059]
- Natural Science Foundation of Shandong Province [ZR2011EMQ010]
- Science and Technology Development Plan of Shandong Province [2014GSF120004]
- Science and Technology Plan Project of Jinan [201307010]
- Special Project for Independent Innovation and Achievements Transformation of Shandong Province [2014ZZCX05101]
- Special Foundation for Taishan Scholar Professorship of Shandong Province
- UJN [ts20130937]
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Detectio of tissue polypeptide antigen (TPA) in human serum plays an important role in monitoring the occurrence of cancers. In this work, a sandwich-type electrochemical immunosensor based on a novel dual signal amplification strategy was employed for quantitative detection of TPA. Multifunctional graphene nanocomposites (Au@MGN) were designed as labels to achieve a high sensitivity and low limit of detection (LOD), which dominated the dual signal amplification strategy. On one hand, introduction of graphene could accelerate the electron transfer capability of magnetic graphene nanocomposites (MGN) than single ferriferrous oxide nanoparticles (Fe3O4 NPs). On the other hand, introduction of gold nanoparticles (Au NPs) could build a synergetic effect between Fe3O4 NPs and Au NPs, which resulted in the increasing of electrocatalytic properties of Au@MGN. In short, due to the excellent electrochemical property of Au@MGN, high electrocatalytic current responses toward the reduction of hydrogen peroxide (H2O2) were achieved. Under optimum conditions, the proposed sandwich-type electrochemical immunosensor exhibited a wide linear range from 10(-5) ng/mL to 10(2) ng/mL with a low LOD of 7.5 fg/mL for TPA. The designed immunosensor displayed an excellent analytical performance with good reproducibility, high selectivity and stability, indicating potential application promising in clinical monitoring of tumor markers. (C) 2015 Elsevier B.V. All rights reserved.
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