Journal
NANO LETTERS
Volume 14, Issue 10, Pages 5740-5747Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl502626s
Keywords
Nanotechnology; structural DNA nanotechnology; DNA origami; lambda DNA; on-chip DNA synthesis
Categories
Funding
- National Science Foundation [CDI-0835794, OISE-1246799, EPMD-1231888]
- MolPhysX program at the University of Copenhagen
- Directorate For Engineering
- Div Of Electrical, Commun & Cyber Sys [1231888] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0835794] Funding Source: National Science Foundation
- Office Of Internatl Science &Engineering
- Office Of The Director [1246799] Funding Source: National Science Foundation
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Structural DNA nanotechnology, and specifically scaffolded DNA origami, is rapidly developing as a versatile method for bottom-up fabrication of novel nanometer-scale materials and devices. However, lengths of conventional single-stranded scaffolds, for example, 7,249-nucleotide circular genomic DNA from the M13mp18 phage, limit the scales of these uniquely addressable structures. Additionally, increasing DNA origami size generates the cost burden of increased staple-strand synthesis. We addressed this 2-fold problem by developing the following methods: (1) production of the largest to-date biologically derived single-stranded scaffold using a lambda/M13 hybrid virus to produce a 51 466-nucleotide DNA in a circular, single-stranded form and (2) inexpensive DNA synthesis via an inkjet-printing process on a chip embossed with functionalized micropillars made from cyclic olefin copolymer. We have experimentally demonstrated very efficient assembly of a 51-kilobasepair origami from the lambda/M13 hybrid scaffold folded by chip-derived staple strands. In addition, we have demonstrated two-dimensional, asymmetric origami sheets with controlled global curvature such that they land on a substrate in predictable orientations that have been verified by atomic force microscopy.
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