Journal
NANO LETTERS
Volume 14, Issue 4, Pages 2065-2070Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl500234t
Keywords
Single molecule detection; label-free; biosensing; myosin 5a; interferometric scattering microscopy
Categories
Funding
- John Fell Fund
- EPSRC [EP/H003541]
- ERC
- National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health [FEB013960]
- CONACyT [213546]
- Marie Curie Fellowship [330215]
- National Heart, Lung, and Blood Institute, National Institutes of Health [ZIA HL004229]
- Electron Microscopy Core Facility of the NHLBI
- EPSRC [EP/H003541/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/H003541/1] Funding Source: researchfish
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Optical detection of individual proteins requires fluorescent labeling. Cavity and plasmonic methodologies enhance single molecule signatures in the absence of any labels but have struggled to demonstrate routine and quantitative single protein detection. Here, we used interferometric scattering microscopy not only to detect but also to image and nanometrically track the motion of single myosin 5a heavy meromyosin molecules without the use of labels or any nanoscopic amplification. Together with the simple experimental arrangement, an intrinsic independence from strong electronic transition dipoles and a detection limit of <60 kDa, our approach paves the way toward nonresonant, label-free sensing and imaging of nanoscopic objects down to the single protein level.
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