Journal
NANO LETTERS
Volume 13, Issue 9, Pages 3967-3972Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl304359p
Keywords
Localization microscopy; dipole emission pattern; molecular orientation; rotational diffusion; localization error
Categories
Funding
- National Science Foundation
- 3Com Corporation Stanford Graduate Fellowship
- Robert and Marvel Kirby Stanford Graduate Fellowship
- National Institute of General Medical Sciences Grant [R01GM085437]
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The asymmetric nature of single-molecule (SM) dipole emission patterns limits the accuracy of position determination in localization-based super-resolution fluorescence microscopy. The degree of mislocalization depends highly on the rotational mobility of SMs; only for SMs rotating within a cone half angle alpha > 60 degrees can mislocalization errors be bounded to <= 10 nm. Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstructions.
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