Journal
NANO LETTERS
Volume 13, Issue 11, Pages 5414-5419Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl4029818
Keywords
Total internal reflection scattering (TIRS); single-particle; orientation determination; microtubule cargo; localized surface plasmon resonance
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Funding
- U.S. Department of Energy, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences, and Biosciences through the Ames Laboratory
- U.S. Department of Energy by Iowa State University [DE-AC02- 07CH11358]
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Single-particle and single-molecule orientation determination plays a vital role in deciphering nanoscale motion in complex environments. Previous attempts to determine the absolute three-dimensional orientation of anisotropic particles rely on subjective pattern matching and are inherently plagued by high degrees of uncertainty. Herein, we describe a method utilizing total internal reflection scattering microscopy to determine the 3D orientation of gold nanorods with subdegree uncertainty. The method is then applied to the biologically relevant system of microtubule cargo loading. Finally, we demonstrate the method holds potential for identifying single particles versus proximate neighbors within the diffraction limited area.
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