Journal
NANO LETTERS
Volume 13, Issue 11, Pages 5672-5678Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl4033523
Keywords
STED microscopy; STED lithography; polymer nanostructures; single protein array; single molecule imaging; STORM imaging
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Funding
- Deutsche Forschungsgemeinschaft (DFG) [KL1432/5-1, SPP 1327]
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Acrylate nanoanchors of subdiffraction-limited diameter are written with optical stimulated emission depletion (STED) lithography. After incubation, 98% of all nanoanchors are loaded quickly with fluorescently labeled antibodies. Controlling the size of the nanoanchors allows for limiting the number of the antibodies. Direct stochastic optical reconstruction microscopy (dSTORM) imaging, statistical distribution of fluorescence, quantitative fluorescence readout, and single molecule blinking consistently prove that 80% of the nanoanchors with a 65 nm diameter are carrying only one antibody each, which are functional as confirmed with live erythrocytes.
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