Journal
NANO LETTERS
Volume 13, Issue 12, Pages 6144-6150Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl403469r
Keywords
alpha-hemolysin; nanopore; RNA sequencing; cyclodextrin; base identification; protein engineering
Categories
Funding
- National Institutes of Health
- Oxford Nanopore Technologies
- Wellcome Trust
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We describe a label-free ribobase identification method, which uses ionic current measurement to resolve ribonucleoside monophosphates or diphosphates in alpha-hemolysin protein nanopores containing amino-cyclodextrin adapters. The accuracy of base identification is further investigated through the use of a guanidino-modified adapter. On the basis of these findings, an exosequencing approach is envisioned in which a processive exoribonuclease (polynucleotide phosphorylase) presents sequentially cleaved ribonucleoside diphosphates to a nanopore.
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