Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 220, Issue -, Pages 1145-1151Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.06.094
Keywords
HER2; Aptamer; Cancer biomarker; Biosensor; Cancer diagnosis
Funding
- Scientific and Technological Research Council of Turkey (TUBITAK) [110E287]
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In this study, a label-free capacitive aptasensor was developed based on capturing of human epidermal growth factor receptor 2 (HER2) protein by anti-HER2 ssDNA aptamers functionalized on interdigitated microelectrodes of capacitor as bio-recognition elements. The aptasensor response was measured by non-Faradaic Impedance Spectroscopy (nFIS) method. Capacitance signal specific to target HER2 protein is based on changes occurred due to charge distribution upon interaction of aptamer-protein molecules against to the applied AC frequency (50-350 MHz). HER2 protein in spiked human serum was successfully detected through concentration dependent changes in impedance/capacitance values as a result of the formation of aptamer-HER2 protein complex (0.2-2 ng mL(-1) of HER2) on capacitor microelectrodes. The label-free capacitive aptasensing is a versatile and promising approach for early detection of cancer biomarkers in dilute human serum and the method potentially be extended to a wide variety of diseases biomarkers. (C) 2015 Elsevier B.V. All rights reserved.
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