Journal
NANO LETTERS
Volume 13, Issue 6, Pages 2500-2505Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl400560r
Keywords
alpha-Hemolysin; RNA; translocation; nanopore; heteropolymer; single-molecule
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Funding
- National Human Genome Research Institute (National Institutes of Health)
- Oxford Nanopore Technologies
- Royal Thai Government
- Thailand National Science and Technology Development Agency
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The electrophoretic translocation of polynucleotides through nanopores may permit direct single-molecule nucleic acid sequencing. Here we describe the translocation of ssRNA heteropolymers (91-6083 bases) through the alpha-hemolysin nanopore. Translocation of these long ssRNAs is characterized by surprisingly long, almost complete ionic current blockades with durations averaging milliseconds per base (at +180 mV). The event durations decrease exponentially with increased transmembrane potential but are largely unaffected by the presence of urea. When the ssRNA is coupled at the 3' end to streptavidin, which cannot translocate through the pore, permanent blockades are observed, supporting our conclusion that the transient blockades arise from ssRNA translocation.
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