Journal
NANO LETTERS
Volume 13, Issue 2, Pages 625-631Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl304209p
Keywords
Nanotube sensor; lysozyme; enzymology; single molecule; field-effect transistors
Categories
Funding
- NCI of the NIH [R01 CA133592-01]
- NSF [DMR-1104629, ECCS-0802077]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1104629] Funding Source: National Science Foundation
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Single-molecule experimental methods have provided new insights into biomolecular function, dynamic disorder, and transient states that are all invisible to conventional measurements. A novel, nonfluorescent single-molecule technique involves attaching single molecules to single-walled carbon nanotube field-effective transistors (SWNT FETs). These ultrasensitive electronic devices provide long-duration, label-free monitoring of biomolecules and their dynamic motions. However, generalization of the SWNT FET technique first requires design rules that can predict the success and applicability of these devices. Here, we report on the transduction mechanism linking enzymatic processivity to electrical signal generation by a SWNT FET. The interaction between SWNT FETs and the enzyme lysozyme was systematically dissected using eight different lysozyme variants synthesized by protein engineering. The data prove that effective signal generation can be accomplished using a single charged amino acid, when appropriately located, providing a foundation to widely apply SWNT FET sensitivity to other biomolecular systems.
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