Journal
NANO LETTERS
Volume 12, Issue 2, Pages 1092-1095Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl204496g
Keywords
Plasmon sensor; single molecule detection; adsorption dynamics; coverage fluctuations
Categories
Funding
- ERC [259640]
- Carl-Zeiss Foundation
- graduate school of excellence Materials Science in Mainz
- European Research Council (ERC) [259640] Funding Source: European Research Council (ERC)
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The ultimate detection limit in analytic chemistry and biology is the single molecule. Commonly, fluorescent dye labels or enzymatic amplification are employed. This requires additional labeling of the analyte, which modifies the species under investigation and therefore influences biological processes. Here, we utilize single gold nanoparticles to detect single unlabeled proteins with extremely high temporal resolution. This allows for monitoring the dynamic evolution of a single protein binding event on a millisecond time scale. The technique even resolves equilibrium coverage fluctuations, opening a window into Brownian dynamics of unlabeled macromolecules. Therefore, our method enables the study of protein folding dynamics, protein adsorption processes, and kinetics as well as nonequilibrium soft matter dynamics on the single molecule level.
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