Journal
NANO LETTERS
Volume 12, Issue 3, Pages 1157-1164Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl3002827
Keywords
Solid-state nanopore; E. coli RNA polymerase; DNA; single-molecule; AFM; protein shape and dipole
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Funding
- European Research Council [259398]
- Swiss SystemsX.ch initiative (IPhD), Swiss National Science Foundation
- FP7 nanoDNA sequencing grant
- European Research Council (ERC) [259398] Funding Source: European Research Council (ERC)
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In the past decade, a number of single-molecule methods have been developed With the aim of investigating single protein and nucleic acid interactions. For the first time we use solid-state nanopore sensing to detect a single E. coli RNAP DNA transcription complex and single E. coli RNAP enzyme. On the basis of their specific conductance translocation signature, we can discriminate and identify between those two types of molecular translocations and translocations of bare DNA. This opens up a new perspectives for investigating transcription processes at the single-molecule level.
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