Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 218, Issue -, Pages 229-236Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.05.006
Keywords
Nitrogen-doped carbon quantum dots (N-CQDs); Fluorescent probe; NADH; Cellular imaging
Funding
- National Natural Science Foundation of China [21175114, 21305067]
- Natural Science Foundation of Jiangsu Province [BK2011441, 20130754]
- PhD Fund of MOE for Young Teachers [0133219120019]
- Qing Lan project of Jiangsu Province, NJUST Research Dounding [2012ZDJH009]
- Fundamental Research Funds for the Central Universities [30920130112012]
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In this work, nitrogen-doped carbon quantum dots (N-CQDs) with high fluorescence quantum yield (46.2%) were successfully synthesized through one-pot hydrothermal carbonization approach assisted by ethylenediamine as surface passivation agent, wherein alanine acted as a single carbon source, ethylenediamine as the surface passivation agent. The obtained biocompatible N-CQDs could effectively label both the MCF-7 cell membranes and the nucleus, suggesting the potential application in biolabeling and bioimaging. Furthermore, the addition of dihydronicotinamide adenine dinucleotide (NADH) could dramatically quench the fluorescence of N-CQDs. Therefore, a novel protocol for NADH determination was established and its mechanism was discussed. This proposed fluorescence sensor displayed an enhanced performance for NADH determination with the linear range up to 80 mu M and the detection limit down to 25.1 nM. (C) 2015 Elsevier B.V. All rights reserved.
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