Journal
NANO LETTERS
Volume 11, Issue 1, Pages 209-213Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl103472b
Keywords
Microscopy; fluorescence; superresolution; localization; resolution; single molecules
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Funding
- Deutsche Forschungsgemeinschaft
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We investigate the cooperative effect of molecular tilt and defocus on fluorophore localization by centroid calculation in far-held superresolution microscopy based on stochastic single molecule switching, If tilt angle and defocus are unknown, the localization contains systematic errors up to about +/-125 nm. When imaging rotation-impaired fluorophores of unknown random orientation, the average localization accuracy in three-dimensional samples is typically limited to about +/-32 nm, restricting the attainable resolution accordingly.
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