Journal
NANO LETTERS
Volume 11, Issue 2, Pages 746-750Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl1038874
Keywords
Alpha-hemolysin; DNA sequencing; nanopore; protein engineering; DNA translocation
Categories
Funding
- National Institutes of Health
Ask authors/readers for more resources
Protein nanopores may provide a cheap and fast technology to sequence individual DNA molecules. However, the electrophoretic translocation of ssDNA molecules through protein nanopores has been too rapid for base identification. Here, we show that the translocation of DNA molecules through the alpha-hemolysin protein nanopore can be slowed controllably by introducing positive charges into the lumen of the pore by site directed mutagenesis. Although the residual ionic current during DNA translocation is insufficient for direct base identification, we propose that the engineered pores might be used to slow down DNA in hybrid systems, for example, in combination with solid-state nanopores.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available