4.8 Article

Functionalized Micromachines for Selective and Rapid Isolation of Nucleic Acid Targets from Complex Samples

Journal

NANO LETTERS
Volume 11, Issue 5, Pages 2083-2087

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/nl2005687

Keywords

Micromachines; nucleic acid; matrix isolation; biological fluids

Funding

  1. National Science Foundation [CBET 0853375]
  2. National Institutes of Health [U01 AI075565]
  3. Programa Becas Complutense del Amo
  4. Scientific and Technical Council of Turkey (TUBITAK)
  5. German Research Foundation (DFG, Heisenberg) [FL 384/7-1]
  6. Directorate For Engineering
  7. Div Of Chem, Bioeng, Env, & Transp Sys [0853375] Funding Source: National Science Foundation

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The transport properties of single-strand DNA probe-modified self-propelling micromachines are exploited for on-the-fly hybridization and selective single-step isolation of target nucleic acids from raw microliter biological samples (serum, urine, crude E. coli lysate, saliva). The rapid movement of the guided modified microrockets induces fluid convection, which enhances the hybridization efficiency, thus enabling the rapid and selective isolation of nucleic acid targets from untreated samples. The integration of these autonomous microrockets into a lab-on-chip device that provides both nucleic acid isolation and downstream analysis could thus be attractive for diverse applications.

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