Journal
NANO LETTERS
Volume 11, Issue 11, Pages 5047-5053Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl203114f
Keywords
DNA; confinement; crowding; nanochannel; nanofluidic; biophysics
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Funding
- Singapore-MIT Alliance for Research and Techology (SMART)
- National Science Foundation [CBET-0852235]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0852235] Funding Source: National Science Foundation
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We experimentally and numerically study the effects of macromolecular crowding agents on DNA structure when confined to a nanochannel. Curiously, DNA response to crowding is significantly different between bulk phase, nanoslit confinement, and nanotube confinement. Coarse grained Brownian dynamics simulations reproduce trends seen in the experiments and allow us to develop a deeper understanding of the key physics at play in these systems. It is proposed that the occupancy of free volume next to the channel wall by crowders causes an effective reduction in confining dimensions of the channel that initially swells DNA in nanoconfinement.
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