Journal
NANO LETTERS
Volume 12, Issue 1, Pages 473-478Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl203876w
Keywords
Evanescent field; TIRF microscopy; point spread function; magnetic tweezers; DNA; Holliday junction
Categories
Funding
- DFG [SE 1646/1-1]
- European Research Council [261224]
- NanoSci-E+ collaborative proposal [SE 1646/5-1]
- Dresden International Graduate School for Biomedicine and Bioengineering
- Engineering and Physical Sciences Research Council [EP/H006702/1] Funding Source: researchfish
- EPSRC [EP/H006702/1] Funding Source: UKRI
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The characterization of three-dimensional inhomogeneous illumination fields is a challenge in modern microscopy. Here we use a four-arm DNA junction as a nanomechanical translation stage to move a single fluorescent quantum dot through an exponentially decaying evanescent field. Recording the emission of the quantum dot within the evanescent field as well as under homogeneous illumination allows one to directly obtain the intensity distribution of the excitation field without additional deconvolution. Our method will allow the characterization of a broad range of illumination fields and to study near-field effects between small optical probes.
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