Journal
NANO LETTERS
Volume 11, Issue 1, Pages 170-175Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl103286k
Keywords
Field-effect transistors; protein interactions; click chemistry; porphyrins; glycoclusters; biosensors
Categories
Funding
- Universite Claude Bernard Lyon 1
- CNRS
- Region Rhone-Alpes (Cluster de Recherche Chimie)
- ANR Glycoasterix
- Vaincre la Mucovisdicose
- GDR Pseudomonas
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We have used single-walled carbon nanotube field-effect transistor (NTFET) devices to probe the interactions between carbohydrates and their recognition proteins called lectins. These interactions are involved in a wide range of biological processes, such as cell-cell recognition, cell-matrix interaction as well as viral and bacterial infections. In our experiments, NTFETs were functionalized noncovalently with porphyrin-based glycoconjugates synthesized using click azide-alkyne chemistry, and change in electrical conductance was measured upon specific binding of two bacterial lectins that present different carbohydrate preference, namely PA-IL, PA-IIL from Pseudomonas aeruginosa and a plant lectin Concanavalin A. However, no significant change in the device characteristics was observed when the devices were exposed to other lectins with different specificity. Detection of PA-IL binding to galactosylated NTFETs was highly sensitive (2 nM) with a measured dissociation constant (K-d = 6.8 mu M) corresponding to literature data Fluorescence microscopy, atomic Force microscopy, UV-vis-NIR spectroscopy, and several control measurements confirmed the NTFET response to selective interactions between carbohydrates and lectins.
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