Journal
NANO LETTERS
Volume 11, Issue 4, Pages 1826-1830Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl2006092
Keywords
Surface plasmon; ELISA; biosensor; single molecules; single particles; spectra imaging
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Funding
- Swedish Research Council through the Linnaeus Center Bioinspired Supramolecular Function and Design (SUPRA)
- Sweden's innovation agency VINNOVA [2010-02762]
- National Science Foundation [EEC-0647560, CHE-0911145, DMR-0520513, BES-0507036]
- National Cancer Institute [1 U54CA119341-01]
- Vinnova [2010-02762] Funding Source: Vinnova
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0911145] Funding Source: National Science Foundation
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Robust but ultrasensitive biosensors with a capability of detecting low abundance biomarkers could revolutionize clinical diagnostics and enable early detection of cancer, neurological diseases, and infections. We utilized a combination of localized surface plasmon resonance (LSPR) refractive index sensing and the well-known enzyme-linked immunosorbent assay to develop a simple colorimetric biosensing methodology with single molecule sensitivity. The technique is based on spectral imaging of a large number of isolated gold nanoparticles. Each particle binds a variable number of horseradish peroxidase (HRP) enzyme molecules that catalyze a localized precipitation reaction at the particle surface. The enzymatic reaction dramatically amplifies the shift of the LSPR scattering maximum, lambda(max), and makes it possible to detect the presence of only one or a few HRP molecules per particle.
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