Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 210, Issue -, Pages 120-123Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2014.10.061
Keywords
Cross-flow filtration; Microfluidic separation; Rapid Antimicrobial Susceptibility Testing; Sepsis; Whole blood bacterial filter
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A microfluidic device to separate bacteria from blood cells based on size-exclusion through cross-flow channels was designed and performance tested using fluorescently-labeled, heat-killed Escherichia coli spiked into whole blood. The device is easy and cheap to fabricate, and simply and robustly purifies bacteria from large blood cells. Thus the device would be an effective sample-preparation stage within a point-of-care system for rapid testing for antibacterial resistance. (C) 2014 Elsevier B.V. All rights reserved.
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