Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 211, Issue -, Pages 1-6Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.01.051
Keywords
Hg2+ detection; Gold nanoparticles; Biosensor; Sensitivity; Selectivity
Funding
- National Natural Science Foundation of China [21303043, 21173071]
- Research Fund for the Doctoral Program of Higher Education of China [20114104110002]
- Program for Innovative Research Team (in Science and Technology) in University of Henan Province [14IRTSTHN005]
- Key Project for Science and Technology Research in Henan Province Department of Education [13B150082]
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We have developed a sensitive, selective, and simple fluorescence spectrometric sensor for Hg2+ detection in aqueous solution by using thiol-DNA-functionalized gold nanoparticles (Au NPs). This sensor is based on the Hg2+-induced conformational change of single-stranded DNA (ssDNA) with an enhanced fluorescence resonance energy transfer ( FRET) process between the energy donor (fluorescein, FAM) and the energy acceptor (Au NPs). In the presence of Hg2+, a formation of a hairpin structure for ssDNA originates from thymine-Hg2+-thymine (T-Hg2+-T) coordination, resulting in notable fluorescence quenchingin comparison to the random coil. In order to improve the stability and sensitivity of the sensor, we adopt short helper oligonucleotides to increase the density of DNA on the surface of Au NPs. The assay enables the detection limit for the detection of Hg2+ is 8 nM, and is extremely specific for Hg2+ even in the presence of high concentrations of other metal ions. Toward the goal for practical applications, this sensor was applied to monitor Hg2+ in tap water samples and showed low matrix interference and high sensitivity. (C) 2015 Elsevier B.V. All rights reserved.
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